PROJECTS
Arbovirus-vector interactions
We have investigated the antiviral role of host small RNA interference pathways in controlling arbovirus replication and spread using high-throughput sequencing as well as cell biological and virological methods (including use of reverse genetics to modify viruses). We have also studied other antiviral responses.
These studies are now being expanded in vitro, to understand the molecular mechanisms underlying these host responses; and in vivo, to understand their mode of action in mosquitoes. We have also begun work to understand the antiviral effects of Wolbachia endosymbionts.
Arbovirus-vertebrate cell interactions
In addition to interactions with vectors and vector cells, we study interactions of arboviruses with vertebrate cells, including primary lines. This line of research also uses the reverse genetics systems and replicons developed for arboviruses, with which we frequently study vector cells.
At present, investigations are focused on Schmallenberg virus interactions with bovine cells; dengue virus interactions with human cells; and Rift Valley fever virus replication. Also work on the recently identified Zika virus. It includes work on immune pathways, but also more fundamental studies on protein interactions and replication mechanisms. We plan to expand this research to other arboviruses.
Collaborators
Prof. John Fazakerley, Dr. Rennos Fragkoudis and Dr. Lesley Bell-Sakyi (Pirbright Institute), Prof. Andres Merits (University of Tartu, Estonia), Prof. Libor Grubhoffer (University of South Bohemia, Czech Republic), Dr. Anna-Bella Failloux (Institut Pasteur, Paris, France), Prof. Richard Elliott (CVR), Dr. Stefanie Becker (FLI, Germany), Dr Andrew Davidson (University of Bristol), Dr Jamel Mankouri (University of Leeds), Dr Andrew Tuplin (University of Leeds), Dr. Esther Schnettler (CVR).
Grants
Grants and Awards listed are those received whilst working with the University of Glasgow.
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US-UK BBSRC-NIFA Collab: Control of emerging bunyaviruses
Biotechnology and Biological Sciences Research Council
2015 - 2018
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Wolbachia as a defence against RNA viruses in insects
Wellcome Trust
2013 - 2014
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Medical Research Foundation Equipment Grant
Medical Research Council
2013 - 2013
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Vector competence of European mosquitoes to Rift Valley fever virus
Biotechnology and Biological Sciences Research Council
2012 - 2015